- ￥12184.64
- ATCC
- KCSP.ATCC.HB-296
- 2025年11月27日
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文献和实验Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
at least twice. If convinced that the subclone is monoclonal, checking the isotype is possible . If not convinced that the hybrydoma is monoclonal, reclone a second time. Cloning of Hybridoma Make an accurate cell count of cells
Immunizing Schedules for Hybridoma Production
Immunization protocols for generating activated B-lymphocytes suitable for hybridoma production vary widely. The optimal amount of antigen given, the way of antigen presentation, and the timing between injections must be determined
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