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文献和实验16 Quantification of Gene Expression in Mouse and Human Renal Proximal Tubules
techniques. We describe protocols that have been used to successfully isolate messenger RNA from proximal tubules of both freshly prepared and archival samples using laser capture microdissection and laser-manipulated microdissection.
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
The kidney is an extremely heterogeneous organ from a morphological and a functional point of view. In spite of this heterogeneity, several methods have been described in an attempt to isolate and culture homogeneous populations of renal
Primary Kidney Proximal Tubule Cells
Primary rabbit kidney epithelial cell cultures can be obtained that express renal proximal tubule functions. Toward these ends, renal proximal tubules are purified from the rabbit kidney by the method of Brendel and Meezan. To summarize
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