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文献和实验Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin
. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.
Isolation and Culture of Skin Fibroblasts
Dermal fibroblasts synthesize and organize skin connective tissue (dermis). Fibroblast cultures provide a powerful tool for investigating both the molecular mechanisms that regulate skin connective tissue production and the pathophysiology
Genetically Modified Skin Substitutes: Preparation and Use
a porous sponge of collagen and glycosaminoglycan (5 ,6 ) and a fibroblast contracted collagen lattice (7 ,8 ). We and others have had success with a dermal analog of acellular human dermis that is deepithelialized and rendered completely acellular
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