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文献和实验Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin
Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials
Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models
The uppermost layer of our skin, the epidermis, is formed largely of keratinocytes which constitute the skin’s major barrier function and the first line of defence against environmental physical, chemical and biological agents. The subsequent
Electroporation of Plasmid DNA into Normal Human Fibroblasts
that are of interest to a particular study. Normal human fibroblast cell strains have a proliferative potential of from 50–75 cumulative population doublings (CPD) (3 ,4 ). Since expansion from one cell to a million cells requires approx 20 population doublings, human fibroblasts
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