- ￥14525.20
- ATCC
- KCSP.ATCC.TIB-217
- 2025年10月28日
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文献和实验Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
Fusion of Mouse, Rat, or Hamster Cell
glutamine (Life Technologies, Inc. #25030-149 or equivalent) HAT (Life Technologies, #31062-0211 or equivalent) Hybridoma Cloning Factor (Fisher # IG50-0615) PEG1500 (Boehringer Mannheim # 0783-641
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
information for VH and VL is generally amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody-specific primers A variety of primer sets for the amplification of mouse-variable domams has been developed that are particulary
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