- ￥12184.64
- ATCC
- KCSP.ATCC.CRL-2993
- 2025年10月12日
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文献和实验Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Primary mouse embryonic fibroblasts (PMEFs) have a number of properties that make them an attractive cell culture model. Relative to other primary explant cultures they are easy to establish and maintain, proliferate rapidly and, as a result
of the House Mouse, Mus Musculus Genome number of chromosomes 40 diploid DNA content about 6 pg per cell 3X109 bp per haploid genome recombination units 1600 centimorgans (2000 Kb/cM) approximate number of genes§ 0.5 - 1.0 X 105 percent of genome
Culturing Mouse Embryonic Fibroblasts
Dissecting tools: Scissors, forceps, scalpel 95% ethanol in a squeeze bottle and in a beaker Mouse breeders Note: This protocol describes the isolation of primary MEFs and passage according to the 3T3 protocol. Passaging primary cells
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