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文献和实验Construction and Characterization of Adenovirus Vectors
Construction and Characterization of Adenovirus Vectors P. Joel Ross and Robin J. Parks Adapted from Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). CSHL Press, Cold Spring Harbor, NY, USA, 2007
Adenovirus Entry into Cells: A Quantitative Fluorescence Microscopy Approach
to the target cell or inefficient DNA import into the nucleus. In this chapter, we describe a quantitative method to determine transport of fluorescently labeled wild-type adenovirus 2 to the nucleus of a model cell line, HeLa cells. This protocol
Analysis of the Efficiency of Adenovirus Transcription
by hybridization of membrane-bound DNA following purification of DNA from nuclear fractions of adenovirus-infected cells. Two alternative protocols, primer extension and quantitative reverse transcription polymerase chain reaction, are described for determination
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