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文献和实验Generation of Lymphoblastoid Cell Lines (LCLs)
Epstein-Barr virus (EBV) is a lymphotropic γ herpes virus. Infection of human B cells with EBV in vitro results in their immortalization and the resulting cell lines are named lymphoblastoid cell lines (LCLs) (1 ). In these cells, EBV
Antibodies for Detecting EBV Latent Proteins
slab gel apparatus giving 16�16 cm gels. Lane 1 contains protein from an EBV-negative B cell line, and lanes 2 through 7 contain proteins from 6 lymphoblastoid cell lines, each transformed with a different EBV isolate. The upper blot was probed
Production of Human Monoclonal Antibodies by the EpsteinBarr Virus Method
Epstein–Barr virus (EBV) is a herpes virus which in vitro efficiently immortalizes nearly all human B lymphocytes. The lymphoblastoid diploid cell lines (LCL’s) thus generated preserve the characteristics of the cells initially infected
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