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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
as the "input" sample for subsequent analysis (see Step 37). Prepare one input sample for each treatment group or cell type. 23. Remove an 800-µL aliquot from the sample (from Step 21). Add it to 7.2 mL of ChIP diluent (i.e., a 10-fold
using AmpliTaqGold®, verify the initial denaturation/activation time of 12 minutes at 95°C. c. For all other hot start methods, confirm the proper use of the reagents. 2. Potential Problem: Experimental DNA samples were degraded
Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
of all even numbered fatty acid peaks with the peak of the internal standard. Specifications for the run: Column type HP-1 Injection temperature 260 °C
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