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文献和实验. The pir gene is usually supplied by a lambda lysogen. Such strains include DH5alpha-lambdapir, SY327-lambdapir, SM10-lambdapir, and S17-lambdapir. The last two strains supply the tra genes for efficient conjugation. Cloning into pCVD442 Cloning in pCVD
Strategies to Optimize Protein Expression in E. coli
, N.P.J., Lilley, P.E., and Dixon, N.E. 1990. Modified bacteriophage‐lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 87:123‐126. Esposito, D
5´ ... G T^A C ... 3´ 3´ ... C A^T G ... 5´ Source: An E. coli strain that carries the cloned Rsa I gene from Rhodopseudomonas sphaeroides (S. Kaplan). Reaction Conditions: (Supplied with enzyme) NEBuffer 1 10 mM Bis Tris Propane-HCl, 10 mM MgCl
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