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文献和实验Strategies to Optimize Protein Expression in E. coli
. Butt, T.R., Jonnalagadda, S., Monia, B.P., Sternberg, E.J., Marsh, J.A., Stadel, J.M., Ecker, D.J., and Crooke, S.T. 1989. Ubiquitin fusion augments the yield of cloned gene‐products in Escherichia coli. Proc
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
-wide analysis of protein binding in mammalian cells. Curr Protoc Mol Biol, 2007. Chapter 21: p. Unit 21 13. [24] Metzger, E., et al., LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription. Nature, 2005. 437(7057): p. 436
Analysis of Protein Co‐Occupancy by Quantitative Sequential Chromatin Immunoprecipitation
Additional reagents and equipment for ChIP (unit 21.3 , Basic Protocol), growth of Saccharomyces cerevisiae (units 13.1 & 13.2 ), extraction and purification of DNA (unit 2.1 ), and real‐time quantitative PCR (unit 21
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