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文献和实验Epstein-Barr Virus Transformation of Lymphoblasts
This protocol describes a method for the transformation of lymphoblasts by Epstein-Barr Virus (EBV). Cells may be isolated from whole blood or taken from cryopreserved, non-immortalized stocks (please see Cryopreservation of Mammalian Culture Cells
The Southern blot technique can be used to determine the clonality of Epstein-Barr virus (EBV) infected cells (1 ,2 ). This clonality assay capitalizes on measurable polymorphisms in EBV genomic structure, namely, the variable number
In situ hybridization (ISH) to Epstein-Barr virus (EBV)-encoded RNA (EBER) is considered the “gold standard” for detecting and localizing latent EBV in biopsy samples. Transcripts from the EBER1 and EBER2 genes are an appropriate target
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