Broadpharm代理商-Octyl azide 7438

Replication timing by density transfer

, then add Pronase (0.01-0.05 mg/ml final conc.). [You can add dry Pronase. I usually dissolve the Pronase in 5 ml of minimal medium, just to avoid having the dry Pronase stick to the sides of the flask.] 5. Hold at 37o C for 90-120 min

Replication timing by comparative hybridization

the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml

DETECTION OF RECOMBINANT PROTEINS BY ELISA

pH to 7.4. QS to 500 ml with water. Dilute 1:10 for final concentration. PBS-Tween (washing solution) PBS plus 0.05% Tween-20, 0.02% sodium azide) 400 ml 10x PBS 8 ml 10% sodium azide