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上海玉博生物科技有限公司
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文献和实验Direct Immunofluorescence Labeling
complex after the antibodies bind to the receptors. Cells in suspension, counted and viability-checked. Keep them in culture medium supplemented with antibiotics and 2-5 fetal bovine serum, on ice. If viability is less than 90, consider adding
Basic Method for Direct Immunofluorescence Labeling
in suspension, counted and viability-checked. Keep them in culture medium supplemented with antibiotics and 2-5% fetal bovine serum, on ice. If viability is less than 90%, consider adding another fluorochrome to identify dead cells during analysis. Equipment
Indirect flow cytometry (FACS) protocol
are difficult to resuspend. It is always useful to check the viability of the cells which should be around 95% not less than 90%. 2. Resuspend the cells to approximately 1-5 x 10 6 cells/ml in ice cold PBS, 10% FCS, 1% sodium azide. Use ice
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