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上海玉博生物科技有限公司
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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
that are not clear, using DMSO instead of glycerol for the first polymerization step appears to increase the labeling stoichiometry by ~25% for C2CF-SNHS ester. For labeling with rhodamine (tetramethylrhodamine NHS ester) and X-rhodamine, we generally use glycerol
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
-1 pH Indicator A special cell-loading technique permits ratiometric measurement of intramitochondrial pH with our SNARF dyes. Cell loading with 10 µM 5-(and 6-)carboxy SNARF-1, acetoxymethyl ester, acetate (C1271, C1272; Section 20.2), followed
Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
‐NHS;Pierce) in 50 mM MES; prepare immediately before use. 0.5 M hydroxylamine hydrochloride 25 mM Tris⋅Cl, pH
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