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上海玉博生物科技有限公司
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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
that are not clear, using DMSO instead of glycerol for the first polymerization step appears to increase the labeling stoichiometry by ~25% for C2CF-SNHS ester. For labeling with rhodamine (tetramethylrhodamine NHS ester) and X-rhodamine, we generally use glycerol
Site‐Specific Protein Labeling with SNAP‐Tags
) Anhydrous N ,N ‐dimethyl formamide (DMF, Sigma‐Aldrich) IRDye 800CW N‐hydroxysuccinimide (NHS) ester (Licor, cat. no. 929–70020; http://www.licor.com
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
that is compatible for high?throughput screening is also provided. Curr. Protoc. Cytom. 51:13.11.1?13.11.15. © 2010 by John Wiley & Sons, Inc. Keywords: RGS; G protein; protein?protein interaction; FCPIA; high?throughput screening; multiplexing
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