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文献和实验Synthesis of Peptide‐Oligonucleotide Conjugates Using a Heterobifunctional Crosslinker
in the assembly of bivalent protein affinity reagents. This unit describes the synthesis and purification of POC molecules using the heterobifunctional crosslinking reagent succinimidyl?4?(N?maleimidomethyl)cyclohexane?1?carboxylate (SMCC), which enables amine
esters, which give off this characteristic smell. This is not a sign of incomplete DEPC removal and it will not interfere with any subsequent reactions. Technical Bulletin 178 ). Also, modification of the reagent's amine groups could affect
Tris[(1‐benzyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]amine (TBTA; Sigma‐Aldrich) Methoxypolyethylene glycol azide 5000 (PEG5000‐N 3 ; Sigma‐Aldrich
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