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文献和实验-nitrophenyl phosphate as substrate. Bone Gla protein(BGP) was measured by radioimmunoassay.Type I collagen,MMP-1,TIMP-1 mRNA was examined using semiquantitive reverse transcription-polymerase chain reaction(RT-PCR) analysis.Human osteosarcoma cell lines MG-63
Transactivation and Transrepression Studies with p53
The methods outlined in this chapter are designed to facilitate the study of the transactivation and transrepression properties of p53 (as well as p63 and p73). Once a gene of interest is identified, its presumptive promoter region
20 mM HEPES, pH 7.5.C. Alkaline Hydrolysis of Run-Off Transcripts1. Add 63 μl of 1 M NaOH to the transcripts and leave on ice for 15 min.2. Add 125 μl 1M HEPES free acid, 50 μl 3M sodium acetate, and 1 ml Ethanol to precipitate the nucleic acids
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