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文献和实验Planar Lipid Bilayer Experiment平面脂双层实验
until warm (room temperature) or moisture will get into vial and hydrate the lipid.Coat the hole in chamber with about 3ul of oxidized cholesterol, let air-dry. For single channel, coat one side; for selectivity, coat both sides. Another lipid commonly used
Phosphate Assay by Suprya Jaydev
% by weight) 971.1 ml H2O Ammonium molybdate stock: 4.2 ml Ammonium molybdate 1000 ml 1N H2SO4 Procedure 1) Prepare standards in duplicate. --> standards: 0, 3, 5, 7, 10, 12, and 15 µl of a 1 mM NaH2PO4 stock solution
Phosphate Assay by Suprya Jaydev
) 880.3 ml H2O 1 N Sulfuric acid stock: 28.9 ml concentrated sulfuric acid (18M/36N @ 96% by weight) 971.1 ml H2O Ammonium molybdate stock: 4.2 ml Ammonium molybdate 1000 ml 1N H2SO4 Procedure 1) Prepare standards in duplicate
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