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文献和实验Metabolic Labeling with Fatty Acids
of radiolabeled fatty acids (e.g., [3 H]myristate or palmitate) is an alternative method for labeling proteins. This unit contains methods for biosynthetic labeling with fatty acids, analysis of the fatty acid linkage with protein, analysis of total protein?bound
Immunohistochemistry: Fluorescence Protocol 2.0
that have been harvested, fixed and permeabilized at the peak of cytokine production after in vitro stimulation with strong polyclonal activators such as phorbol 12-myristate 13 acetate/ionomycin, anti-CD3+ CD28 monoclonal antibody, LPS or bacterial superantigens
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