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文献和实验TRICINE GELS Protocol (For Low MW proteins (
Stock Solutions1) Gel Buffer: 3 M TrisHCl pH8.45 + 0.3% SDS. Keep RT. (Prepare: 18.15gr Tris + 150mg SDS + H2O up to 50ml. Adjust pH before you add the SDS) 2) 40% Acrylamide solution 29:1 (Acrylamide 29 / Methylene bis Acrylamide 1) or 40
DETECTION OF RECOMBINANT PROTEINS BY ELISA
volume with carbonate coating buffer (pH 9.6) to coat the ELISA plate. Two plates can be coated with the 1 times the volume, 4 plates with 2 times the volume. Label 96 well Costar (#2797) plates as needed. (See attached example of a 96 well plate
part of the procedure and unfortunately it is the most critical as well. Wash the cells once in sorbitol buffer and suspend in 100-500ul of the same. BE VERY VERY GENTLE! Place on ice. Coat the wells of teflon coated slides with 0.1 polylysine (>400,000 MW
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