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文献和实验Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
How to Make Simple Solutions and Dilutions
unit volumes of broth (990 ul) = 1:100 dilution. In the second step, one unit volume of the 1:100 dilution is combined with 99 unit volumes of broth now yielding a total dilution of 1:100x100 = 1:10,000 dilution. Repeated again (the third step
Isolation of Microtubules (Bovine Brain)
curve. Decant the remainder of the crude supernatant into a chilled beaker, and add an equal volume of 8 M glycerol in MT buffer. Add dry GTP (MW 523) to the crude supernatant to make a 1.0 mM final concentration
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