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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 4.3 mM CaCl2,1.2 M KI,50 mM Tris-HCl,pH 8.0,5 ml. 5.0.5 mM ATP,0.2 mM CaCl2,0.5 mM DTT,2 mM Tris-HCl,pH 8.0 at 4℃,1 liter. 6.CFSE (Molecular Probes,C-1311; not the diacetate),prepare a stock solution of 50 mg/ml in DMSO. 7.Small SS34 tubes and adaptors
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
An important approach in studies of normal, diseased, and malignant cells is their growth in culture. The isolation and subsequent culture of human epidermal melanocytes has been attempted since 1957 (1 -5 ), but only since 1982 have pure
Site‐Specific Protein Labeling with SNAP‐Tags
/) EZ‐Link NHS‐PEG 12 ‐Biotin (Thermo Scientific) Phosphate‐buffered saline (PBS; appendix 2E ) Cell
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