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文献和实验Purification of Plasmid from 50 ml-culture
is visible at the bottom of the tube, vortex well to dissolve the pellet. Incubate the tube at 37 C for further 30 min. 16. Add 240 ul of 2M NaCl, 20% PEG8000. (PEG6000 supplied from Japanese suppliers is essentially equivalent to PEG8000, and works
and incubate at 37 ℃ for 20 to 30 minutes to digest remaining RNA.11. Precipitate the plasmid DNA with PEG solution (30% polyethylene glycol, 1.6 M NaCl) by adding 0.4 ml and incubating 1hr-overnight on ice. This step discriminates very large plasmid DNA
In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
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