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文献和实验in salt solutions containing 8 M urea, whereas the cell cytoplasm and the majority of nuclear components, except H3/H4, are washed away. This protocol yields highly purified H3/H4 in a few minutes without the use of chromatography steps. The acid
Acid Phosphatase for Glycol Methacrylate Sections Procedure
, sodium salt (Sigma) - 40 mg N.N-dimethylformamide - 4 ml Pararosaniline Solution Pararosaniline (C.I.# 42500) - 2 g 2N HCl - 50 ml Use heat to dissolve, filter when cool and store at 4°C. Sodium Nitrite Solution
Acid Phosphatase for Glycol Methacrylate Sections Procedure
Naphtol AS-BI phosphatease, sodium salt (Sigma) - 40 mg N.N-dimethylformamide - 4 ml Pararosaniline Solution Pararosaniline (C.I.# 42500) - 2 g 2N HCl - 50 ml Use heat to dissolve, filter when cool and store at 4°C
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