Product Overview The NEXTFLEX® Small RNA-Seq Kit v4 with UDIs can be used to prepare small RNA libraries from total RNA or purified small RNA and is designed to greatly reduce formation of adapter-dimer product in small RNA-Seq library preparation, allowing for complete gel-free library preparation from typical input amounts. This kit is designed to greatly reduce sequence bias in small RNA sequencing library construction, allowing more accurate identification and quantification of microRNAs, piRNAs, and other small RNAs.
Sequencing libraries generated by this kit contain Unique Dual Indices (UDIs), which are designed to specifically address the index-hopping phenomenon associated with Illumina platforms utilizing a patterned flow cell. These UDIs prevent mis-assigned reads from appearing in final datasets, allowing for the highest assurance of data integrity. These indexes work on Illumina® instruments such as the MiSeq®, HiSeq® 2000/2500, MiniSeq®, NextSeq®, and NovaSeq®.
Kit Overview This kit can be used to generate 8, 48, or 96 small RNA libraries. The kit contains the necessary reagents to process the user’s purified RNA sample through library preparation.
Kit Contents, Storage & Shelf Life The NEXTFLEX® Small RNA-Seq Kit v4 with UDIs contain enough material to prepare 8, 48, or 96 RNA samples for Illumina-compatible next-generation sequencing. The shelf life of all reagents is at least 6 months when stored properly. The Nuclease-free Water and Resuspension Buffer can be stored at room temperature. The NEXTFLEX® Cleanup Beads should be stored at 4°C, and all other components should be stored at -20°C.