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上海经科化学科技有限公司
MEM(PM150410)+10% FBS(164210)+1% P/S(PB180120)
人
1×10⁶cells/T25培养瓶
名称 | Daoy (人髓母细胞瘤细胞) (STR鉴定正确) |
别称 | DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia. The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. |
年龄(性别) | 男性;4岁 |
组织来源 | 结缔组织增生性小脑髓母细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 神经母细胞瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | 33.6 hours, at 48th passage (PubMed=2993532); 34 hours (ATCC) |
致瘤性 | Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.) |
保藏机构 | ATCC; HTB-186 BCRJ; 0325 |
Amelogenin | X |
CSF1PO | 11 |
D2S1338 | 16,17 |
D3S1358 | 15 |
D5S818 | 11,13 |
D7S820 | 8,10 |
D8S1179 | 13,15 |
D13S317 | 13,14 |
D16S539 | 10 |
D18S51 | 12 |
D19S433 | 14,14.2 |
D21S11 | 29,31.2 |
FGA | 23 |
PentaD | 10,13 |
PentaE | 7,11 |
TH01 | 9 |
TPOX | 8,10 |
vWA | 14,20 |
D6S1043 | 12 |
D12S391 | 20 |
D2S441 | 10,11 |
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