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- 详细信息
- 文献和实验
- 技术资料
- CAS号:
无
- 保质期:
参考说明
- 保存条件:
常温
- 英文名:
Ca Ski
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
1×10⁶cells/T25培养瓶
| 名称 | Ca Ski (人宫颈癌肠转移细胞/人宫颈癌上皮细胞) (STR鉴定正确) |
| 别称 | Ca-Ski; CaSki; Caski; CASKI |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
| 培养方案A(默认) |
生长培养基:
RPMI-1640+10% FBS+1% P/S
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | Ca Ski细胞是从小肠肠系膜转移灶的细胞中建立的。据报道,Ca Ski细胞含有完整的HPV-16(每个细胞大约600个拷贝)和HPV-18相关序列。 |
| 年龄(性别) | 女性;40岁 |
| 组织来源 | 宫颈癌肠转移 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 宫颈癌细胞 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~60-80 hours |
| 基因表达情况 | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
| 保藏机构 | ATCC; CRL-1550 ATCC; CRM-CRL-1550 ATCC; CRL-7915BCRC; 60251 ECACC; 87020501 |
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 21 D3S1358 15 D5S818 13 D7S820 8,11 D8S1179 15 D13S317 8,12 D16S539 11,12 D18S51 17 D19S433 15,16 D21S11 30 FGA 21 PentaD 10 PentaE 5 TH01 7 TPOX 8 vWA 17 D6S1043 11,14 D12S391 18 D2S441 11 -
STR鉴定图
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