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武汉华美生物工程有限公司
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Indirect
ELISA
Homo sapiens (Human)
serum, plasma
96T/48T
规格: | 96T | 产品价格: | ¥3600.0 |
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规格: | 48T | 产品价格: | ¥2500.0 |
The human parathyroid hormone 1-34 (PTH 1-34) antibody ELISA kit allows for the qualitative identification of PTH 1-34 antibodies in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with the PTH 1-34. Samples or standards are pipetted into the wells with anti-human immunoglobulin conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human PTH 1-34 antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The PTH 1-34 antibody titer in the samples is determined by referring to the negative control. It indicates the presence of PTH 1-34 antibody if the O.D. (optical density) of PTH 1-34 antibody is greater than or equal to the cutoff value(2.1× Average O.D. value of negative control). There is no PTH 1-34 antibody present in the sample if the O.D. is less than the cutoff value.
This assay has excellent specificity for the detection of PTH 1-34 antibodies. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.
PTH 1-34 is a type of parathyroid hormone approved for the treatment of osteoporosis in postmenopausal women and in men who are at high risk of breaking a bone. It contributes to rebuilding bone and significantly increasing bone mineral density.
Intra-assay Precision (Precision within an assay): CV%<15% | |||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||||
Test parameter | specification | test result | |||||||
Positive control | >0.8 | 1.895 | |||||||
Negative control | <0.2 | 0.135 | |||||||
Positive rate | 10,Positive | 100% | |||||||
Negative rate | 10,Negative | 100% | |||||||
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华美生物ELISA试剂盒受到全球数千名研究人员的信赖,并引用到了许多优秀的高分期刊上,如:Nature,Science, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology 等。
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