The product CSB-EL021135HU is a sandwich ELISA kit developed to measure levels of human 14-3-3 protein sigma (SFN) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of SFN bound in the initial step.
SFN is a negative regulator of the cell cycle and has been identified as a human mammary epithelium-specific marker downregulated in transformed mammary carcinoma cells. It has also been regarded as a p53-inducible gene product involved in cell cycle checkpoint control after DNA damage. Studies have shown that overexpression of SFN inhibited oncogene-activated tumorigenicity in a tetracycline-regulated SFN system. SFN expression has also shown potential in therapeutic intervention in cancers.
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