These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料:
An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
Biotin-labeled NT-proBNP antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
Four Adhesive Strips(For 96 wells) --- Seal the microtiter plate when incubation.
An instruction manual
本试剂盒不含材料:
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution
货期:3-5 working days
研究进展:NT-proBNP,全名为N末端B型利钠肽前体,是一种由心脏细胞合成的激素分子。最近的研究表明,NT-proBNP可以作为心衰的标志物,可用于心衰的诊断、预测和治疗监测。此外,NT-proBNP还参与了心血管疾病、肾脏疾病和糖尿病等多种疾病的发生和发展。
Midregional pro-atrial natriuretic peptide is a superior biomarker to N-terminal pro-B-type natriuretic peptide in the diagnosis of heart failure patients with preserved ejection fraction.Cui.et al.Medicine.2018.Comparative symptom biochemistry between moderate and advanced heart failure.Lee CS.et al.heart & lung.2018.Natriuretic peptides and echocardiographic parameters in Mexican children environmentally exposed to arsenic.JM Torres-Arellano.Toxicology and Applied Pharmacology.2020.A Synergistic Effect between Plasma Dickkopf-1 and Obstructive Coronary Artery Disease on the Prediction of Major Adverse Cardiac Events in Patients with Angina: An Observational Study.YH Li.Biomolecules.2022.Impact of dapagliflozin on cardiac function following anterior myocardial infarction in non-diabetic patients–DACAMI (a randomized controlled clinical trial).KA Dayem.International journal of cardiology.2023.Hitting the (bio) mark part 1: selecting and measuring biomarkers in cardiovascular research.AA Khaled.European journal of cardiovascular nursing..