This dog IL10 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of dog IL10 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL10 levels in the sample and is measured at 450 nm through a microplate reader.
IL10 is an anti-inflammatory cytokine that is produced by many hematopoietic cells and mainly functions to inhibit macrophage and dendritic cell (DC) functions, which are necessary for the capture, control, and initiation of immune responses to pathogens. IL10 both impedes pathogen removal and improves immunopathology. IL-10 suppresses the expression of cytokines such as TNF-α, IL-1β, and IL-8, and can repress the expression of adhesion molecules. In addition, IL-10 can deactivate monocytes by downregulating the expression of MHC surface molecules. IL10 also plays a role in the control of allergy and asthma.