手机验证
500
武汉华美生物工程有限公司
0.156 ng/mL-10 ng/mL
Sandwich
ELISA
Rattus norvegicus (Rat)
serum, plasma, cell culture supernates, tissue homogenates
96T/48T
规格: | 96T | 产品价格: | ¥3600.0 |
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规格: | 48T | 产品价格: | ¥2500.0 |
This Rat Alox5 ELISA Kit was designed for the quantitative measurement of Rat Alox5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat Alox5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 96 | |||||
Range % | 92-100 | ||||||
1:2 | Average % | 102 | |||||
Range % | 95-106 | ||||||
1:4 | Average % | 90 | |||||
Range % | 83-94 | ||||||
1:8 | Average % | 93 | |||||
Range % | 88-97 |
The recovery of rat Alox5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 90 | 84-95 | |||||
EDTA plasma (n=4) | 100 | 92-105 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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