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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉华美生物工程有限公司
- 检测范围:
34.3 pg/mL-2200 pg/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Rattus norvegicus (Rat)
- 标记物:
Non-conjugated
- 样本:
serum, plasma, tissue homogenates, cell lysates
- 灵敏度:
8.5 pg/mL
- 规格:
48T
产品类型:
ELISA Kit英文名称:
Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit中文名称:
大鼠NAD依赖性蛋白脱乙酰酶sirtuin-1(SIRT1)酶联免疫试剂盒货号:
CSB-EL021339RA规格:
96T/48T价格:
¥3600/¥2500别名:
RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1缩写:
SIRT1Uniprot No.:
/种属:
Rattus norvegicus (Rat)样本类型:
serum, plasma, tissue homogenates, cell lysates检测范围:
34.3 pg/mL-2200 pg/mL灵敏度:
8.5 pg/mL反应时间:
1-5h样本体积:
50-100ul检测波长:
450 nm研究领域:
Epigenetics and Nuclear Signaling测定原理:
quantitative测定方法:
Sandwich货期:
3-5 working days靶点名称:
sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)请点击“在线询价”找客服索取产品说明书及引用文献目录,更多文献信息可以查看cusabio官网3w.cusabio.cn/references.html
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文献和实验Sirtuins are a class of enzymes with important functions in regulation aging, metabolism, and genome stability. They were originally known as nicotinamide adenine dinucleotide (NAD)-dependent protein lysine deacetylases. However, recently
In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
Evaluating the precise catalytic activity of sirtuin proteins in vivo is a challenging endeavor. Enzymological methods, including those employed in commercially available kits, require the isolation of immunopurified protein from cells
使用:乳酸是葡萄糖不完全氧化的产物。研究表明,体外培养条件下95%的葡萄糖转变为乳酸,这降低了营养物质的代谢效率,降低培养基pH值,增加渗透压。在氧气供给不足的情况下,NADH转运系统苹果酸-天冬氨酸穿梭系统活性低而不能将糖酵解产生的NADH氧化磷酸化为NAD+,细胞只得以降低能量需求的方式如激活乳酸脱氢酶将糖酵解产生的丙酮酸与NADH反应生产乳酸和NAD+,从而保证了糖酵解的顺利进行。另一个可能的解释是连接糖酵解与TCA循环的特异性酶如丙酮酸脱氢酶复合物、磷酸丙酮酸羧化酶激酶和丙酮酸羧化
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