手机验证
500
武汉华美生物工程有限公司
0.78 pg/mL-50 pg/mL
Sandwich
ELISA
Rattus norvegicus (Rat)
serum, plasma, cell culture supernates, tissue homogenates
96T/48T
规格: | 96T | 产品价格: | ¥3900.0 |
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规格: | 48T | 产品价格: | ¥2500.0 |
The Rat Stromal cell-derived factor 1a (SDF-1a) ELISA Kit is a reliable and efficient solution for researchers in the field of immunology. This ELISA kit enables the quantitative measurement of SDF-1a in a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates from Rattus norvegicus (Rat).
With a detection range of 0.78 pg/mL to 50 pg/mL and a sensitivity of 0.195 pg/mL, this ELISA kit delivers accurate and precise results, making it a valuable tool for researchers working on SDF-1a in rats. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.
This ELISA kit works on a sandwich assay principle, where the SDF-1a antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.
The Rat SDF-1a ELISA Kit has been cited in more than 4 publications, highlighting its reliability and utility in research. It is an excellent addition to any immunology research laboratory, providing accurate and precise measurement of SDF-1a in rat samples.
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 88 | ||||
Range % | 83-97 | |||||
1:200 | Average % | 97 | ||||
Range % | 91-103 | |||||
1:400 | Average % | 84 | ||||
Range % | 80-88 | |||||
1:800 | Average % | 89 | ||||
Range % | 81-95 |
The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 89-96 | ||||
EDTA plasma (n=4) | 97 | 94-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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