The human NLRP3 ELISA Kit allows for the in vitro quantitative determination of NLRP3 concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes human NLRP3. NLRP3 inflammasome is a multi-protein complex containing a sensor NLRP3 protein, an adaptor apoptosis-associated speck-like protein (ASC), and the zymogen procaspase-1. It is a critical component of the innate immune system that mediates the activation of caspase-1 and the secretion of proinflammatory cytokines IL-1β/IL-18 in response to microbial infection and cellular damage. The NLRP3 inflammasome is involved in the modulation of numerous physiological and pathological processes, such as infections, tissue repair, autoimmune diseases, and cancer. Aberrant activation of the NLRP3 inflammasome has been linked with several inflammatory disorders, which include cryopyrin-associated periodic syndromes (CAPS), Alzheimer's disease, diabetes, and atherosclerosis.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. NLRP3 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled NLRP3 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with NLRP3 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
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