Gene Name: CST3 Source: Mouse Clonality: Monoclonal Isotype: IgG2a Species Reactivity: human Tested Application: WB, IHC, ICC/IF, FCM, ELISA(Cap) Contents: 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. Immunogen: E. coli-derived human Cystatin C recombinant protein (Position: K31-A146). Concentration: 500 ug/ml Purification: protein G purified. MW: 16 kDa Dilution Ratios: Western blot (WB): 1:500-2000 Immunohistochemistry (IHC): 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow Cytometry (Fixed): 1:50-200 ELISA(Cap): 1:50-1:200 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. Storage:12 months from date of receipt,-20°C as supplied. 6 months 2 to 8°C after reconstitution. Avoid repeated freezing and thawing. Background:Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.