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- 96-0056C15
- 2025年10月31日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Vapor phase of liquid nitrogen
- 保质期：
unknown
- 英文名：
InCELL Hunter™ A549 BTK Kinase Binding Cell Line
- 库存：
99
- 供应商：
北京孚博
- CAS号：
96-0056C15
- 规格：
1 cryovial
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文献和实验Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells
. A subset of these assays monitors mitogen-activated protein (MAP) kinase pathways. MAP kinases are principal regulators of fundamental processes in mammalian cells, including growth, cell division, differentiation, stress responses, and neoplastic
A general protocol for staining cell for cytometry analysis
-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1× Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 13024D) into 100 µl of 1× Binding Buffer and add to the cell pellet. Gently mix the cells. Add
the GE InCell 3000 automated imaging platform. The assay utilized a genetically modified HeLa cell line stably expressing mitogen-activated, protein-activating protein kinase-2 fused to enhanced green fluorescent protein (MK2–EGFP) and measured
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