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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Vapor phase of liquid nitrogen
- 保质期:
unknown
- 英文名:
PathHunter® HEK 293 CD27 NF-kB Signaling Pathway Reporter Cell Line
- 库存:
99
- 供应商:
北京孚博
- CAS号:
93-1166C1
- 规格:
1 cryovial
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文献和实验Using Reporter Gene Technologies to Detect Changes in cAMP as a Result of GPCR Activation
). This chapter provides a generic protocol for the successful execution of a reporter gene assay for GPCRs, stably transfected within Chinese hamster ovary cell lines, signaling through the Gs pathway. It also highlights areas to investigate when developing
v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
over control conditioned media-treatedcells. B A monoclonal HEK293T cell line stably expressing pBARVS was generated as described in Section 3.4 . Cells weretreated with either control conditioned media or Wnt3a-conditioned media for 30 h.Control reporters
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
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