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2.细胞培养生长迅速，倍增时间短、克隆效率高
3.无病毒及支原体污染，内毒素水平低
4.对标澳洲胎牛血清，适合部分原代、干细胞等难养细胞的培养与扩增
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Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Culture of BEND Cells (Bovine Endometrial Cells)
Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse serum (H1138) Antibiotic-antimycotic (ABAM) (A9909) D-valine (V1255) Insulin (I5500) Preparation 1. Place 1000 ml of double distilled water
TUNEL PROCEDURE IN BOVINE EMBRYOS
) to the remaining 450 µl Label Solution in bottle 2 to obtain 500 µl TUNEL reaction mixture. Mix well to equilibrate components. Note: The TUNEL reaction mixture should be prepared immediately before use and should not be stored.
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