Influenza A H1N1 Hemagglutinin / HA Antibody, Rabbit MAb

产品名称Influenza A H1N1 Hemagglutinin / HA Antibody, Rabbit MAb
经验证的应用WB (FAQProtocol), ELISA (FAQProtocol), ELISA(Det) (FAQProtocol)
WB (FAQProtocol), ELISA (FAQProtocol), ELISA(Det) (FAQProtocol)
相关说明IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特异性
H1N1 (A/Puerto Rico/8/1934) HA
Has cross-reactivity in WB with
H3N2 (A/Darwin/9/2021) HA Protein (Cat# 40859-V08H)
H3N2 (A/Darwin/9/2021) HA Protein (Cat# 40859-V08B)
H3N2 (A/Darwin/6/2021) HA Protein (Cat# 40868-V08H)
No cross-reactivity in WB and ELISA with
Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein (Cat# 40862-V08H)
Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein (Cat# 40862-V08B)

No cross-reactivity in ELISA with
H1N1 (A/Brevig Mission/1/1918) HA
H1N1 (A/Brisbane/02/2018) HA
H1N1 (A/California/04/2009) HA
H1N1 (A/California/07/2009) HA
H1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HA
H1N1 (A/Michigan/45/2015) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/Victoria/4897/2022) HA Protein (Cat# 40938-V08H)
H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HA Protein (Cat# 40787-V08H)
H1N1 (A/Wisconsin/67/2022) HA Protein (Cat# 40940-V08H)
H2N2 (A/Canada/720/2005) HA
H3N2 (A/Brisbane/10/2007) HA
H3N2 (A/Cambodia/e0826360/2020) HA
H3N2 (A/Darwin/9/2021) HA Protein (Cat# 40859-V08H)
H3N2 (A/Darwin/9/2021) HA Protein (Cat# 40859-V08B)
H3N2 (A/Darwin/6/2021) HA Protein (Cat# 40868-V08H)
H3N2 (A/Darwin/6/2021) HA Protein (Cat# 40868-V08B)
H3N2 (A/Hong Kong/2671/2019) HA
H3N2 (A/Hong Kong/45/2019) HA
H3N2 (A/Hong Kong/4801/2014) HA
H3N2 (A/Kansas/14/2017) HA
H3N2 (A/Singapore/INFIMH-16-0019/2016) HA
H3N2 (A/Switzerland/9715293/2013) HA
H4N4 (A/mallard/Ohio/657/2002) HA
H5N1 (A/Anhui/1/2005) HA
H6N1 (A/northern shoveler/California/HKWF115/2007) HA
H7N7 (A/chicken/Netherlands/1/03) HA
H8N4 (A/pintail duck/Alberta/114/1979) HA
H9N2 (A/Hong Kong/1073/99) HA
H10N3 (A/duck/Hong Kong/786/1979) HA
H11N2 (A/duck/Yangzhou/906/2002) HA
H12N5 (A/green-winged teal/ALB/199/1991) HA
H13N8 (A/black-headed gull/Netherlands/1/00) HA
H15N8 (A/duck/AUS/341/1983) HA
H16N3 (A/black-headed gull/Sweden/5/99) HA
Influenza B (B/Brisbane/60/2008) HA
Influenza B (B/Colorado/06/2017) HA
Influenza B (B/Florida/4/2006) HA
Influenza B (B/Florida/4/2006) HA
Influenza B (B/PHUKET/3073/2013) HA Protein (Cat# 40498-V08B)
Influenza B (B/Washington/02/2019) HA Protein (Cat# 40722-V08H)
免疫原Recombinant Influenza A H1N1 HA / Hemagglutinin protein (Catalog#11684-V08H)
制备方法This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H1N1 HA / Hemagglutinin (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528).
来源Monoclonal Rabbit IgG Clone #107
纯化Protein A
缓冲液0.2 μm filtered solution in PBS
偶联物Unconjugated
状态Liquid
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H1N1 Hemagglutinin / HA Antibody, Rabbit MAb 经验证的应用

应用	推荐稀释比/用量
WB	1:500-1:2000
ELISA	1:5000-1:10000
ELISA(Det)	1:1000-1:10000

备注
ELISA(Det)：    This antibody will detect Influenza A H1N1 HA / Hemagglutinin in ELISA pair set (Catalog: # SEKA11684). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11684-R016).
请注意：最佳浓度/稀释度应由用户决定。

Influenza A H1N1 Hemagglutinin / HA Antibody, Rabbit MAb 图片


Anti-HA rabbit monoclonal antibody at 1:1000 dilution.
Sample: Recombinant Protein 10 ng
Lane A: H3N2 (A/Darwin/9/2021) HA Protein (Cat# 40859-V08H)
Lane B: H3N2 (A/Darwin/9/2021) HA Protein (Cat# 40859-V08B)
Lane C: H3N2 (A/Darwin/6/2021) HA Protein (Cat# 40868-V08H)
Lane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein (Cat# 40862-V08H)
Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein (Cat# 40862-V08B)
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.

Hemagglutinin/HA 背景信息
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
全称
Harvey rat sarcoma viral oncogene homolog