- ￥3171
- QIAGEN
- 76506
- 德国
- 2025年11月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
RNAprotect Bacteria Reagent (2x100ml)
- 保质期：
一年
- 供应商：
科邦兴业北京科技有限公司
- 保存条件：
常温
- 规格：
kit
qiagen 凯杰一级签约代理商 76506RNAprotect Bacteria Reagent (2x100ml)
RNAprotect Bacteria Reagent
Features
- Immediate stabilization of RNA and RNA protection
- Convenient and safe handling at room temperature
- Easy collection and storage of tissues in reclosable tubes
- Stabilization of Gram-positive and Gram-negative bacteria
- No need to remove medium from sorted or cultured cells
Product Details
RNAprotect Reagents and Tubes provide immediate RNase inactivation and stabilization of RNA in tissues, cultures of Gram-positive and Gram-negative bacteria and sorted or cultured cells to preserve the gene expression profile and enable reproducible purification of high-quality RNA. Samples are stabilized at room temperature prior to RNA isolation procedures.
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文献和实验Large Scale Plasmid Preps: Qiagen/Cesium Method
transfections of mammalian cells. PROCEDURE: Grow 250 - 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4ºC for 10 min. Purify the plasmid
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
of 0.1 ml 1.5 M TCA. Centrifuge the reaction mixture at room temperature (10,000 rpm for 5 min) to remove the precipitate. Determine the ammonia released in the supernatant colorimetrically (A480 ) by adding 0.25 ml Nessler reagent into tubes
How to Make Simple Solutions and Dilutions
of a bacterial culture (see figure below) in the process of quantifying the number of viable bacteria in a culture (see figure below). Each step in this example uses a 1 ml total volume. The initial step combines 1 unit volume of bacterial culture (10 ul) with 99
