- ￥3000
- LDN
- FC E-3100
- 法国
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京博蕾德生物科技有限公司
- 库存：
1000
- 样本：
组胺
- 适应物种：
食品
- 应用：
食品组胺测定
- 检测方法：
ELISA
- 规格：
96T
Cat#FC E-3100
Fish meal that has been produced from materials which has been allowed to degrade prior to being
processed can contain high levels of histamine and can be toxic. Elevated histamine levels (1,000 ppm)
can cause gizzard erosion and black vomit in poultry. Histamine testing in fresh fish is a possible control
strategy that can be used by seafood processors in their HACCP program to address the hazard of
scombrotoxin formation. Histamine is a product of decomposition of histidine caused by the growth of
certain bacteria in seafood. The amount of the amine that forms is a function of bacterial species, the
temperature and time of exposure and may exceed 1,000 ppm (mg/kg). Fish containing high levels of
histamine has been associated with many examples of poisoning commonly referred to as “scombroid
poisoning”, a major health problem for consumers. Scombrotoxic fish usually contains levels of histamine
in excess of 200 ppm, but such fish may be randomly dispersed within a lot. For large fish, histamine is
found at variable levels even within individual fish. Quality control measures designed to minimize the
occurrence of scombrotoxic fish require the determination of histamine levels in the range of
approximately 10 to 200 ppm. Good quality fish contain less than 10 ppm histamine, a level of 30 ppm
indicates significant deterioration, and 50 ppm is considered to be evidence of definite decomposition.
The defect action level (DAL), the level at which regulatory actions are taken for histamine is 50 ppm (P.
L. Rogers, W. F. Staruszkiewicz, Journal of Aquatic Food Product Technology, Vol. 9 (2) 2000 p. 5 – 17).
The assay kit provides materials for the quantitative determination of derivatized histamine in food
extracts. The derivatization is part of the preparation of the samples. By use of the acylation reagent,
histamine is quantitatively derivatized into N-acylhistamine. The competitive Histamine Food ELISA kit
uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated
histamine and solid phase bound histamine compete for a fixed number of antibody binding sites. When
the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing.
The antibody bound to the solid phase histamine is detected by anti-goat/peroxidase. The substrate
TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase
histamine is inversely proportional to the histamine concentration of the sample.
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文献和实验Product Technology 9 (2), 5–17
2. J.M. Hungerford, 2010. Scombroid poisoning: a review. Toxicon 15, 231–243
3. S. Köse, N. Kaklikkaya, S. Koral, B. Tufan, K.C. Buruk, F. Aydin, 2011. Commercial test kits and the
determination of histamine in traditional (ethnic) fish products-evaluation against EU accepted HPLC
method. Food Chemistry, 125 (4) 1490–149
，支原体DNA会显示为特异性条带，以此指示支原体的存在。PCR法可以检测大多数支原体，但谨慎起见最好同时使用另一种检测方法来进行验证。 普健生物生产的支原体PCR检测试剂盒（Mycoplasma PCR Test Kit）是一款具有快速、灵敏、特异性高等特点的支原体检测试剂盒，可用于细胞、培养基、动物血清等的支原体检测。本产品通过聚合酶链式反应技术（PCR）特异扩增支原体16s-rRNA 基因保守区域片段进行支原体检测，能够检测包括M. arthritidis， M
Oxford Biomedical Research的氧化应激及慢性炎症生物标志物检测
准确和可靠（比色法灵敏度为250 nm/荧光法为10 nm），省略了可产生生色团从而影响检测的用强酸煮沸样品的步骤，在室温下操作即可，且省时-仅需15分钟。可检测生物、保健品或食品样品。 1. TBARS比色法检测试剂盒（微孔板），货号FR40，96孔板 2. 改良的TBARS检测（比色皿），货号FR35，100个反应 3. TBARS荧光法检测试剂盒（微孔板），货号FR45，96孔板 尿液掺入或不掺10 uM 丙二醛的检测
上显然会出现排异反应，所以如何对鼠源抗体进行人源化是抗体工程中的一大重点。 此外，利用单抗进行诊断试剂盒开发也是单抗应用的一大重点。目前食品安全是全球关注的一大问题，检测动植物食品中的药物残留、重金属残留以及微量元素的残留，除了传统的HPLC、原子吸收等方法外，试剂盒由于其快速简便、特异性高而越来越受到青睐。主要包括ELISA KIT、WB KIT、胶体金试纸条等诊断检测试剂盒的研制。 2、抗原的设计合成。该话题在园子里已经讨论过多次了，对于小分子抗体制备来说，抗原的设计与合成非常重要
