Mesothelin Recombinant Rabbit mAb(bsm-60005R)-50ul/100ul/25ul

Mesothelin Recombinant Rabbit

mAb(bsm-60005R)-50ul/100ul/25ul
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  • ¥800 - 2500
  • Bioss已认证
  • bsm-60005R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    • 规格

      50ul/100ul/25ul

    规格:50ul产品价格:¥1400.0
    规格:100ul产品价格:¥2500.0
    规格:25ul产品价格:¥800.0
    产品编号bsm-60005R
    英文名称Mesothelin Recombinant Rabbit mAb
    中文名称间皮素重组兔单抗
    英文别名MSLN; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Human skin tumor, Human skin tumor, Human skin tumor, Human skin tumor
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human Mesothelin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量64 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Tumor biomarkers > Tumor antigens

    Cancer > Tumor immunology > Tumor-associated antigens

    Tags & Cell Markers > Cell Type Markers > Epi / Endo-thelial

    Tags & Cell Markers > Cell Type Markers > Tumor Associated

    亚基Interacts with MUC16.
    亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.
    Megakaryocyte-potentiating factor: Secreted.
    Isoform 3: Secreted.
    组织特异性Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    翻译后修饰Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    相似性Belongs to the mesothelin family.
    功能Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    Mesothelin Recombinant Rabbit
    Blocking buffer: 5% NFDM/TBST
    Primary ab dilution: 1:2000
    Primary ab incubation condition: 2 hours at
    room temperature
    Lysate: HeLa
    Protein loading quantity: 20 μg
    Exposure time: 60 s
    Predicted MW: 69 kDa
    Observed MW: 40, 69 kDa
    Mesothelin Recombinant Rabbit
    P‌‌araformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Mesothelin Recombinant Rabbit
    P‌‌araformaldehyde-fixed, paraffin embedded (Human skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mesothelin) Monoclonal Antibody, Unconjugated (bsm-60005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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