Sample:
Lane 1: Mouse Lung tissue lysates
Primary: Anti-FCGR1A/CD64 (bs-25232R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 62 kDa
- ￥1180 - 2800
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- bs-25232R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-25232R |
| 英文名称 | FCGR1A/CD64 Rabbit pAb |
| 中文名称 | 免疫球蛋白G Fc段受体I |
| 英文别名 | High affinity immunoglobulin gamma Fc receptor I; CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; Fc fragment of IgG, high affinity Ia, receptor(CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high affinity Ib, receptor(CD64); Fc fragment of IgG, high affinity Ib, receptor for; Fc fragment of IgG, high affinity Ic, receptor(CD64); Fc fragment of IgG, high affinity Ic, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma receptor I; Fc gamma receptor I B2; Fc gamma RI; Fc of IgG high affinity Ia receptor; Fc-gamma RIC; FCG 1; FCG1; FcgammaRI; FCGR 1; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FCRIC; IGFR 1; IGFR1; IGFRB; IGFRC; IgG Fc receptor I; IgG Fc receptor IC; Immunoglobulin G Fc receptor I; Immunoglobulin G Fc receptor IB; Immunoglobulin G Fc receptor IC. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Mouse, RAW246.8 |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse FCGR1A/CD64 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 41 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Cell Type Markers > CD > Myeloid Cells Immunology > Immunoglobulins > Heavy Chain > IgG Immunology > Innate Immunity > Fc Receptors |
| 亚基 | Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G. |
| 组织特异性 | Monocyte/macrophage specific. |
| 相似性 | Belongs to the immunoglobulin superfamily. FCGR1 family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Blank control（black line）:Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD64 antibody (bs-25232R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat Anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CD64 antibody (bs-25232R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat Anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
. Retrieved 2010-11-29. ^ Nielsen BS, Jørgensen S, Fog JU, Søkilde R, Christensen IJ, Hansen U, Brünner N, Baker A, Møller S, Nielsen HJ (October 2010). "High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival
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FCGR1A/CD64 Rabbit pAb(bs-25232R)-50ul/100ul/200ul
￥1180 - 2800
