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- bs-25013R
- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-25013R |
| 英文名称 | ATG5/APG5L Rabbit pAb |
| 中文名称 | 自噬蛋白5/细胞凋亡的特异性蛋白抗体 |
| 英文别名 | APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ATG5/APG5L |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 32 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Apoptosis > Metabolism Cancer > Cell Death > Apoptosis > Phagocytosis Cancer > Cell Death > Autophagy > APG gene products Cancer > Cell Death > Autophagy > Signal Transduction Cancer > Signal transduction > Autophagy Cardiovascular > Heart > Autophagy > APG gene products Cell Biology > Apoptosis > Intracellular > Associated Proteins Cell Biology > Apoptosis > Phagocytosis Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ub-like Proteins Metabolism > Pathways and Processes > Metabolism processes > Apoptosis Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy > APG gene products |
| 亚基 | The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate. |
| 亚细胞定位 | Cytoplasm. Note=Colocalizes with nonmuscle actin. |
| 组织特异性 | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| 翻译后修饰 | Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. |
| 相似性 | Belongs to the ATG5 family. |
| 功能 | Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-ATG5/APG5L (bs-25013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 50 kDa
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文献和实验[IF={{ 9.8 }}] {Bohan Chen. et al. Inhalation of ammonia promotes apoptosis and induces autophagy in hepatocytes via Bax/BCl-2 and m-TOR/ATG5/LC-3bII axes. SCI TOTAL ENVIRON. 2024 Feb;912:169036} {WB} {Mouse}
[IF={{ 4.2 }}] {Yongjian Chen. et al. Thiram exposure: Disruption of the blood-testis barrier and altered apoptosis-autophagy dynamics in testicular cells via the Bcl-2/Bax and mTOR/Atg5/p62 pathways in mice. PESTIC BIOCHEM PHYS. 2024 Aug;203:106010} {WB,IHC} {Mouse}
[IF={{ 3.9 }}] {Cuicui Zhuang. et al. Escherichia coli infection induces ferroptosis in bovine mammary epithelial cells by activating the Wnt/β-catenin pathway-mediated mitophagy. MITOCHONDRION. 2024 Sep;78:101921} {WB} {Bovine}
[IF={{ 3.8 }}] {Qiuxiang Cai. et al. Pasteurella multocida causes liver injury in ducks by mediating inflammatory, apoptotic and autophagic pathways. MICROB PATHOGENESIS. 2023 Sep;:106336} {WB,IF} {Duck}
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