Cell line: MCF7
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60493M
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- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60493M |
| 英文名称 | WNT2 Recombinant Mouse mAb |
| 英文别名 | Int 1 related protein; IRP protein; WNT 2; INT1L 1; INT1L1; ONCOGENE INT1 LIKE 1; Wingless type MMTV integration site family member 2; WNT2_HUMAN. |
| 产品应用 | WB=1:500-2000, ICC/IF=1:50-200 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Mouse |
| 亚型 | IgG1, Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Tumor suppressors > PTC & Wnt pathway Cancer > Signal transduction Stem Cells > Signaling Pathways > Wnt > Secreted |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed in brain in the thalamus, in fetal and adult lung and in placenta. |
| 相似性 | Belongs to the Wnt family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:50-200} |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate:HeLa, 293T, 4T1, Rat brain
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate:HeLa, 293T, 4T1, Rat brain
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
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WNT2 Recombinant Mouse mAb(bsm-60493M)-50ul/100ul/25ul
￥800 - 2500
