Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60398M
- ￥800 - 2500
- Bioss
- bsm-60398M
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
MEK7 Rabbit pAb(bs-1979R)-50ul/100ul/200ul
￥1180
MAGT1 Rabbit pAb, AP conjugated(bs-18633R-AP)-100ul
￥2980
FBXO9 Rabbit pAb(bs-13165R)-50ul/100ul/200ul
￥1180WWOX Rabbit pAb, BF555 conjugated(bs-0589R-BF555)-100ul
￥2980
Anti-human CLEC7A (Baylor patent Anti-Dectin-1 Biosimilar)(BIO0148SM)-1mg/5mg/20mg
￥3200
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60398M |
| 英文名称 | RAB3GAP1 Recombinant Mouse mAb |
| 产品应用 | WB=1:500-1000, ICC/IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse) |
| 抗体来源 | Mouse |
| 亚型 | IgG1, Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Organogenesis > Nervous system development Neuroscience > Neurology process > Neurogenesis Neuroscience > Neurotransmission > Secretory Vesicles > Rabs Neuroscience > Sensory System > Visual system Signal Transduction > Protein Trafficking > Vesicle Transport > Regulation Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Regulators |
| 亚基 | The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2 |
| 亚细胞定位 | Cytoplasm. Note=In neurons, it is enriched in the synaptic soluble fraction. |
| 组织特异性 | Ubiquitous. |
| 相似性 | Belongs to the Rab3-GAP catalytic subunit family. |
| 功能 | RAB3GAP1 is a member of the RAB3 protein family which are implicated in regulated exocytosis of neurotransmitters and hormones. RAB3GAP, which is involved in regulation of RAB3 activity, is a heterodimeric complex consisting a 130-kD catalytic subunit and a 150-kD noncatalytic subunit. RAB3GAP specifically converts active RAB3-GTP to the inactive form RAB3-GDP (Aligianis et al., 2005 [PubMed 15696165]. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab 3 GAP p130, also known as Rab3 GTPase-activating protein catalytic subunit, is a 981 amino acid protein that belongs to the Rab3-GAP catalytic subunit family. Rab 3 GAP p130 converts active RAB3-GTP to the inactive form RAB3-GDP, and is required for normal eye and brain development. Defects in Rab 3 GAP p130 are the cause of Warburg micro syndrome 1 (WARBM1). WARBM1 is a severe autosomal recessive disorder characterized by developmental abnormalities of the eye and central nervous system and by microgenitalia. The Rab 3 GAP p130 protein may participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. Existing as two alternatively spliced isoforms, the Rab 3 GAP p130 gene is conserved in chimpanzee, dog, cow, mouse, chicken, zebrafish and fruit fly, and maps to human chromosome 2q21.3. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-1000} |
| {ICC/IF} | {1:100-500} |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate:1: HeLa, 2: HepG2, 3:HEK-293, 4:EL4.IL-2
Protein loading quantity: 20 μg
Exposure time: 30s
Predicted MW: 54 kDa
Observed MW: 54 kDa
Primary ab dilution: 1:2000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate:1: HeLa, 2: HepG2, 3:HEK-293, 4:EL4.IL-2
Protein loading quantity: 20 μg
Exposure time: 30s
Predicted MW: 54 kDa
Observed MW: 54 kDa
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
RAB3GAP1 Recombinant Mouse mAb(bsm-60398M)-50ul/100ul/25ul
￥800 - 2500
