Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60461M
- ￥800 - 2500
- Bioss
- bsm-60461M
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60461M |
| 英文名称 | KIF20A Recombinant Mouse mAb |
| 中文名称 | KIF20A单克隆抗体 |
| 英文别名 | KI20A_HUMAN; Kinesin-like protein KIF20A; MKLP2; RAB6KIFL; RCM6; GG10_2; Mitotic kinesin-like protein 2(MKlp2); Rab6-interacting kinesin-like protein; Rabkinesin-6; kinesin family member 20A; |
| 产品应用 | WB=1:1000-5000, ICC/IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse) |
| 抗体来源 | Mouse |
| 亚型 | IgG2b/Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 理论分子量 | 100 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Division > Cytokinesis Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Motor Proteins > Kinesin-like |
| 亚细胞定位 | Golgi apparatus (By similarity). Cytoplasm, cytoskeleton, spindle. |
| 翻译后修饰 | Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle. |
| 相似性 | Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain. |
| 功能 | Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables protein kinase binding activity. Involved in microtubule bundle formation; midbody abscission; and regulation of cytokinesis. Located in several cellular components, including cleavage furrow; intercellular bridge; and midbody. Implicated in restrictive cardiomyopathy. [provided by Alliance of Genome Resources, Apr 2022] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {ICC/IF} | {1:100-500} |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: JK, 2: N2a
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 100 kDa
Observed MW: 120 kDa
Primary Ab dilution: 1:5000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: JK, 2: N2a
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 100 kDa
Observed MW: 120 kDa
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KIF20A Recombinant Mouse mAb(bsm-60461M)-50ul/100ul/25ul
￥800 - 2500
