Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60421M
- ￥800 - 2500
- Bioss
- bsm-60421M
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60421M |
| 英文名称 | Siglec-9 Recombinant Mouse mAb |
| 产品应用 | ICC/IF=1:100-500, Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 亚型 | IgG1, Kappa |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Recombinant |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood Immunology > Cell Type Markers > CD > Adhesion Tags & Cell Markers > Cell Type Markers > Other Cell Types |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon. |
| 相似性 | Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Two families of mammalian lectin-like adhesion molecules bind glycoconjugate ligands in a sialic acid-dependent manner: the selectins and the sialoadhesins. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members recognizing sialylated ligands. The common sialic acids of mammalian cells are N-acetyl-neuraminic acid (Neu5Ac) and N-glycolyl-neuraminic acid (Neu5Gc). Siglec-1 mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Siglec-7, highly expressed in monocytes and resident blood cells, but not in parenchymatous cells, mediates inhibition of natural killer cell cytotoxicity. Siglec-9 is closely homologous to Siglec-7; the gene encoding it maps to chromosome 19q13.41 in humans. It is highly expressed in peripheral blood leukocytes (but not eosinophils), liver, bone marrow, placenta and spleen. Siglec-8, a type I membrane protein, is selectively expressed on human eosinophils, basophils and mast cells, where it regulates their function and survival. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
| {Flow-Cyt} | {1:50-100} |
Specimen: PBMC
Fixative: Unfixed
Permeabilization: None
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Mouse IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Mouse monoclonal IgG1 (Blue line).
Comment: Line red is the positive signal for bsm-60421M
Fixative: Unfixed
Permeabilization: None
Primary Ab dilution: 1:100
Secondary Ab: Goat anti Mouse IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Mouse monoclonal IgG1 (Blue line).
Comment: Line red is the positive signal for bsm-60421M
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Siglec-9 Recombinant Mouse mAb(bsm-60421M)-50ul/100ul/25ul
￥800 - 2500
